• <nav id="ykksy"><nav id="ykksy"></nav></nav>
  • 歡迎訪問上海玉博生物科技有限公司網站

    返回首頁|聯系我們

    全國統一服務熱線:

    021-51875103

    產品中心您當前的位置:首頁 > 產品中心 > 進口試劑 > Enzo life sciences > *,現貨DPPIV drug discovery kit Biomol/BML-AK499

    基礎信息Product information

    產品名稱:DPPIV drug discovery kit Biomol/BML-AK499

    產品型號:*,現貨

    更新時間:2017-04-25

    產品簡介:

    DPPIV drug discovery kitEnzo lifescience BML-AK499;二肽基肽酶 4 /二肽基肽酶IV 檢測試劑盒;*,現貨供應!

    產品特性Product characteristics

    DPPIV drug discovery kit    Enzo lifescience BML-AK499;二肽基肽酶 4 /二肽基肽酶IV  檢測試劑盒,*,現貨供應!

    The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, providing enough material to perform at least 96 assays. DPPIV (DPP4, CD26) is a member of the class of proteases known as prolyl peptidases, which cleave proteins after proline residues and is thought to play roles in diabetes, cancer, and autoimmune diseases, making it a target for drug discovery.

    The kit contains both a chromogenic substrate (H-Gly-Pro-pNA; Km=114 μM) and a fluorogenic substrate (H-Gly-Pro-AMC; Km=50 μM).

    Cleavage of the p-nitroaniline (pNA) from the colorimetric substrate increases absorbance at 405 nm. The fluorimetric assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) moiety from the C-terminus of the peptide substrate, which increases its fluorescence intensity at 460 nm. The kit is useful to screen inhibitors of DPPIV, a potential therapeutic target. A DPPIV inhibitor, P32/98 (KI=130 nM12), is included for use as a control. Other DPP enzymes are available for specificity profiling.

    Product Specification

    Alternative Name:CD26 drug discovery kit, Dipeptidyl Peptidase IV drug discovery kit, DPP4 drug discovery kit
     
    Long Term Storage:-80°C
     
    Kit/Set Contains:DPPIV Enzyme (Human, recombinant) (Prod. No. BML-SE434-9090) (35 mU (40.4μl); 0.867 mU/μl. On U=1μmole/min@37°C, 100μM H-Gly-Pro-pNA. Purity ≥95% (SDS-Page)) Storage: -70°C; avoid freeze thaw cycles
    pNA substrate (Prod. No. BML-PI188-9090) (150μl; 10mM in DMSO) Storage: -70°C
    Calibration standard (p-nitroaniline) (Prod. No. BML-KI106) (1ml; 50μM in assay buffer) Storage: -70°C
    AMC Substrate (Prod. No. BML-P189-9090) (150μl; 0.5mM in DMSO) Storage: -70°C

     

    Calibration standard (7-Amino-4-methylcoumarin) (Prod. No. BML-KI107) (1ml; 40μM in assay buffer) Storage: -70°C)
    Inhibitor (Prod. No. BML-PI142-9090) (20μl; 1mM in DMSO) Storage: -70°C
    Assay Buffer (50mM TRIS, pH 7.5) (Prod. No. BML-KI342) (20ml; liquid in screw-cap plastic bottle) Storage: -20 or -70°C
    ½-volume clear microplate (Prod. No. BML-K101)Storage: Room temperature

    相關文獻:

    Product Literature References

    16, 17-Dihydro-17b-hydroxy isomitraphylline alkaloid as an inhibitor of DPP-IV, and its effect on incretin hormone and β-cell proliferation in diabetic rat: A. Shukla, et al.; Eur. J. Pharm. 47, 512 (2012), Application(s): Measurement of inhibition of DPPIV by DHIM, Abstract;

    Discovery and preclinical profile of Saxagliptin (BMS-477118): a highly potent, long-acting, orally active dipeptidyl peptidase IV inhibitor for the treatment of type 2 diabetes: D. J. Augeri et al.; J. Med. Chem. 48, 5025 (2005), Abstract;

    CD26/dipeptidyl peptidase IV and its role in cancer: B. et al.; Histol. Histopathol. 19, 1345 (2004), Abstract;

    CD26/dipeptidyl peptidase IV: a regulator of immune function and a potential molecular target for therapy: U. Aytac et al.; Curr. Drug. Targets Immune Endocr. Metabol. Disord. 4, 11 (2004), Abstract;

    Dipeptidyl peptidase IV inhibitors for the treatment of diabetes: A. E. Weber et al.; J. Med. Chem. 47, 4135 (2004),Abstract;

    N-linked glycosylation of dipeptidyl peptidase IV (CD26): effects on enzyme activity, homodimer formation, and adenosine deaminase binding: K. Aertgeerts et al.; Protein Sci. 13, 145 (2004), Abstract;

    Dipeptidyl peptidase IV (CD26) activity in the hematopoietic system: differences between the membrane-anchored and the released enzyme activity: D. A. Pereira et al.; Braz. J. Med. Biol. Res. 36, 567 (2003), Abstract;

    Dipeptidyl peptidase IV inhibitor treatment stimulates beta-cell survival and islet neogenesis in streptozotocin-induced diabetic rats: J. A. Pospisilik et al.; Diabetes 52, 741 (2003), Abstract;

    Prolyl peptidases: a serine protease subfamily with high potential for drug discovery: J. S. Rosenblum et al.; Curr. Opin. Chem. Biol. 7, 496 (2003), Abstract;

    Structural basis of proline-specific exopeptidase activity as observed in human dipeptidyl peptidase-IV: R. Thoma et al.; Structure 11, 947 (2003), Abstract;

    On the regulatory role of dipeptidyl peptidase IV (=CD=adenosine deaminase complexing protein) on adenosine deaminase activity: I. Ben-Shooshan; Biochim. Biophys. Acta. 1587, 21 (2002), Abstract;

    Human serum dipeptidyl peptidase IV (DPPIV) and its unique properties: H. Shibuya-Saruta; J. Clin. Lab. Anal. 10, 435 (1996), Abstract;

    Activity of dipeptidyl peptidase IV and post-proline cleaving enzyme in sera from osteoporotic patients: H. Gotoh et al.; Clin. Chem. 34, 2499 (1988), Abstract;

     

    ========================

    二肽基肽酶(dipeptidyl peptidase ,DPP)

    二肽基肽酶(dipeptidyl peptidase ,DPP)是蛋白酶家族中的脯基肽酶成員,其可剪切含脯氨酸的蛋白,其中DPPIV (DPP4, CD26),是一種絲氨酸二肽基肽酶,其剪切靶多肽(細胞趨化因子如CXCL11和肽類激素如GLP-1N端的X-Ala or X-ProCD26是一種高度保守、分布于多種細胞的,具有跨膜域和很短的胞質域,但常被剪切和釋放作為可溶性環化4型,常形成自身二聚體或與FAP形成異二聚體。DPP的天然底物多種多樣,在體內執行多種重要功能。它還是細胞膜受體和共刺激分子,從而參與機體的免疫調節、細胞移行、細胞黏附和細胞調亡過程,故與多種疾病的發病有關。各種DPP-IV抑制劑對II型糖尿病、自身免疫性疾病及細胞增殖性疾?。[瘤)的治療具有重要意義。DPP-4是重要的藥物開發靶點,其中DPP-IV抑制劑Januvia磷酸西他列汀Sitagliptin  phosphate已作為抗糖尿病藥物上市。

    DPP相關產品:(biomol

    Catalog #

    Name

    Category

    size

    AK-499  

    DPPIV Drug Discovery Kit

     DPP  Assay Systems

    96T

    SE-527  

    DPP8 (human, recombinant)

     DPP Enzymes and Assay Systems
    • Prolyl Peptidase Enzymes and Assay Systems

    10 μg

    SE-528  

    DPP9 (human, recombinant)

     DPP Enzymes and Assay Systems
    • Prolyl Peptidase Enzymes and Assay Systems

    10 μg

    SE-564  

    DPPII/DPP7 (human, recombinant)

     DPP Enzymes and Assay Systems
    • Prolyl Peptidase Enzymes and Assay Systems

    10 μg

    SE-529  

    DPPIII (human, recombinant)

     DPP Enzymes and Assay Systems
    • Prolyl Peptidase Enzymes and Assay Systems

    10 μg

    SE-434  

    DPPIV (human, recombinant)

     DPP Enzymes and Assay Systems
    • Prolyl Peptidase Enzymes and Assay Systems

    10 μg

    SE-409  

    FAP (human, recombinant)

     DPP Enzymes and Assay Systems
    • Prolyl Peptidase Enzymes and Assay Systems

    10 μg

    SE-545  

    POP/PREP (human, recombinant)

     DPP Enzymes and Assay Systems
    • Prolyl Peptidase Enzymes and Assay Systems

    10 μg

    P188-0025

    GP-pNA, Chromogenic Substrate   

    DPP 底物

    25mg

    P189-0005 

    GP-AMC, Fluorogenic Substrate 2

    DPP 底物

    5mg

    SA451-0100 

    Anti-DPPIV

    DDP抗體

    100 μg

    SA-472

    Anti-FAPα

    DDP抗體

    100 μg

    SE479-0100 

    Cystatin C

    DPP Inhibitors抑制劑

    100 μg

    EI300-0001 

    EI300-0005 

    GM6001

    DPP Inhibitors抑制劑

    1 mg

    5 mg

    PI142-0010 

    PI142-0050 

    P32/98

    DPP Inhibitors抑制劑

    10 mg

    50 mg

    PI112-0005 

    PI112-0025 

    Z-Prolyl-prolinal

    DPP Inhibitors抑制劑

    5 mg

    25 mg

    EI106-0010 

    EI106-0050 

    Baicalein

    DPP Inhibitors抑制劑

    10 mg

    50 mg

    留言框

    • 產品:

    • 您的單位:

    • 您的姓名:

    • 聯系電話:

    • 常用郵箱:

    • 省份:

    • 詳細地址:

    • 補充說明:

    • 驗證碼:

      請輸入計算結果(填寫阿拉伯數字),如:三加四=7

    上一篇:cygnus 代理

    下一篇:sigma G2761-25MGgly-pro-7-amido-4-methyl-coumarin hydrobromide 原裝進

    上海玉博生物科技有限公司(www.zjstu.com.cn)主營:GIBCO胎牛血清,hyclone血清,標準品,對照品,abcam抗體,進口抗體,sigma試劑,Roche試劑,millipore試劑,Merck試劑

    傳真:86-021-4006768800-5599

    郵箱:ybiotech@163.com

    地址:上海周家嘴路3850號晟鴻商務樓308-310

    ©2021 上海玉博生物科技有限公司 All Rights Reserved.   備案號:滬ICP備10014834號-4  管理登陸  技術支持:化工儀器網  GoogleSitemap

    在線客服 聯系方式 二維碼

    服務熱線

    021-60490116

    021-51875103

    掃一掃,關注我們

    掃一掃訪問手機站掃一掃訪問手機站
    訪問手機站